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octet  (Sartorius AG)


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    Structured Review

    Sartorius AG octet
    Octet, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 8612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/article/Octet/custom%40octet%4042479900
    Average 99 stars, based on 8612 article reviews
    octet - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Binding Assay:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Ubiquitin Proteomics:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Analysis:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Labeling:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Cell Culture:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Construct:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Western Blot:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Enzyme-linked Immunosorbent Assay:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Sequencing:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Transfection:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Negative Control:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Centrifugation:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Control:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Recombinant:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Microscopy:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Incubation:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Comparison:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Virus:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)

    Transduction:

    Article Title: Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8 + T-cell activation.
    Article Snippet: , pB2M-IL2v, pB3-wtIL2, and pB3-IL2v. All constructs were validated by Sanger sequencing (HZYKANG Biotech, Hangzhou, China). HEK 293T cells were transfected AR TIC LE IN PR ES S ARTICLE IN PRESS using jetPRIME (Polyplus, Strasbourg, France, #101000027). At 48 h, supernatants and lysates were assayed by Western blot and ELISA to identify high-expressing constructs (see Method Details for parameters)



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